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Plasmid copy number : ウィキペディア英語版 | Plasmid copy number
Plasmids must regulate their copy number (average number of plasmid copies per cell) to ensure that they do not excessively burden the host or become lost during cell division. Plasmids may be either high copy number plasmids or low copy number plasmids; the regulation mechanisms between these two types are often significantly different. Biotechnology applications may involve engineering plasmids to allow a very high copy number. For example, pBR322 is a low copy number plasmid (~20 copies/cell) that has been adapted into very high copy number plasmid cloning vector (~1000 copies/cell). ==Regulation== High copy number plasmids, also called relaxed plasmids, require a system to ensure that replication is inhibited once the number of plasmids in the cell reaches a certain threshold. Low copy number plasmids, also called stringent plasmids, require tighter control of replication. Relaxed plasmids are generally regulated through one of two mechanisms: antisense RNA or iteron binding groups.
抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「Plasmid copy number」の詳細全文を読む
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